Publications

PUBLICATIONS




*: These authors have contributed equally to this work.

Peer-reviewed original articles


2018

 

Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp V (2018) Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis Cell Systems

doi: 10.1016/j.cels.2018.08.011

 

Pircs K, Petri R, Madsen S, Brattås PL, Vuono R, Ottosson RD, St-Amour I, Hersbach AB, Matusiak-Brückner M, Hult Lundh S, Petersén Å, Déglon N, Hébert SS, Parmar M, Barker AR, Jakobsson J (2018) Huntingtin aggregation impairs autophagy leading to Argonaute-2 accumulation and global microRNA dysregulation Cell Reports

doi: 10.1016/j.celrep.2018.07.017

In this paper we report that aggregation of the mutant huntingtin protein, a hallmark of Huntington’s disease proteinopathy, impairs macroautophagy leading to Argonaute-2 accumulation and global dysregulation of microRNAs. These results indicate that autophagy not only influences protein aggregation, but also directly contributes to the global alterations of post-transcriptional networks in Huntington’s disease. Press release

 

Shrigley S, Pircs K, Barker AR, Parmar M, Drouin-Ouellet J (2018) Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts. J. Vis. Exp.

doi: 10.3791/56904

In this paper and video journal we describe a single vector-based method to generate induced neurons (iNs) from dermal fibroblasts obtained from adult human donors. Since the publishing date 5th February, 2018, the video has already been viewed over 1400 times. I was involved in all of the different experiments, the writing and also performed the FACS sorting chapter in the video. I would like to highlight that this paper has been published without the contribution of my current supervisor (Dr. Johan Jakobsson) during my Postdoc years.

 

2017

 

Drouin-Ouellet J*, Lau S*, Brattås PL, Rylander Ottosson D, Pircs K, Grassi D, Collins ML, Vuono R, Sjöland AA, Westergren-Thorsson G, Graff C, Minthon L, Toresson H, Barker AR, Jakobsson J, Parmar M (2017) REST suppression mediates neural conversion of adult human fibroblasts via microRNA dependent and independent pathways EMBO Molecular Medicine.

doi: 10.15252/emmm.201607471

In this paper we developed an optimized one-step method to efficiently reprogram adult human fibroblasts using a single-vector system. We also demonstrate that it is possible to obtain iNs of high yield and purity from aged individuals with a range of familial and sporadic neurodegenerative disorders including Parkinson's, Huntington's (HD), as well as Alzheimer's disease. I have performed all the relevant data using iNs derived from an HD patient after optimization of culture conditions. I have also helped in setting up and optimizing the iN purification experiments using FACS sorting.

 

Petri R, Pircs K, Jönsson ME, Akerblom M, Brattås PL, Klussendorf T, Jakobsson J. (2017) let-7 regulates radial migration of new-born neurons through positive regulation of autophagy. Embo Journal.

doi: 10.15252/embj.201695235.

This paper is my first paper produced during my Postdoc years in Sweden, where I introduced autophagy to the group. I have designed, performed and analyzed all autophagy related experiments including TEM, immunostainings, rescue data with TFEB and Becn1. Altogether, we have revealed a miRNA-dependent link between autophagy and adult neurogenesis with implications for neurodegenerative diseases where these processes are impaired.

 

2015

 

Takáts Sz, Varga Á, Pircs K, Juhász G. (2015) Loss of Drosophila Vps16A enhances autophagosome formation through reduced TOR activity Autophagy.

doi:  10.1080/15548627.2015.1059559

 

2014

 

Takats Sz*, Pircs K*,Nagy P, Varga A, Karpati M, Hegedus K, Kramer H, Kovacs A, Sass M, Juhasz G. (2014) Interaction of the HOPS complex with Syntaxin 17 mediates autophagosome clearance in Drosophila Mol Biol Cell.

doi.org/10.1091/mbc.e13-08-0449

 

Nagy P, Hegedus K, Pircs K, Varga A, Juhasz G. (2014) Different effects of Atg2 and Atg18 mutations on Atg8a and Atg9 trafficking during starvation in Drosophila FEBS Letters.

doi.org/10.1016/j.febslet.2013.12.012

 

Nagy P*, Karpati M*, Varga A, Pircs K, Venkei Zs, Takats Sz, Varga K, Erdi B, Hegedus K, Juhasz G. (2014) FIP200 promotes phagophore assembly at perilysosomal p62/Ref(2)P aggregates by activation of Atg1 in Drosophila Autophagy.

doi.org/10.4161/auto.27442

 

2013

 

Nagy P, Pircs K, Varga A, Hegedus K, Juhász G. (2013) Myc-driven overgrowth requires unfolded protein response-mediated induction of autophagy and antioxidant responses in Drosophila melanogaster. PloS Genetics.

doi.org/10.1371/journal.pgen.1003664

 

Low P*, Varga A*, Pircs K, Nagy P, Szatmari Zs, Sass M, Juhasz G. (2013) Impaired proteasomal degradation enhances autophagy via hypoxia signaling in Drosophila. BMC Cell Biology.

doi.org/10.1186/1471-2121-14-29

 

Takats Sz, Nagy P, Varga A, Pircs K, Karpati M, Varga K, Kovacs A, Hegedus K, Juhasz G. (2013) Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila. J Cell Biol.

doi: 10.1083/jcb.201211160

This paper is my most cited one (112 cites) where we revealed the molecular mechanism underlying autophagosomal fusion events and show that lysosomal degradation and recycling of sequestered autophagosome content is crucial to maintain proper functioning of the nervous system. I performed in this project all of the immunostainings and analyzed the microscopy data and performed statistical analysis too.

 

2012

 

Pircs K, Nagy P, Varga A, Venkei Z, Erdi B, Hegedus K, Juhasz G. (2012) Advantages and limitations of different p62-based assays for estimating autophagic activity in Drosophila. PloS One.

doi.org/10.1371/journal.pone.0044214

This paper is the first of my first author papers. We compared different experimental approaches for using p62 assays in Drosophila larvae. We produced a p62 antibody in-house, which has been highly cited and commonly used in the fly field ever since. We have also highlighted the advantages and limitations of commonly used p62 assays, which have been applicable also to other cells and organisms used in autophagy research.

 

Erdi B, Nagy P, Zvara A, Varga A, Pircs K, Menesi D, Puskas LG, Juhasz G. (2012) Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila. Autophagy.

doi.org/10.4161/auto.20069

 

Research review articles

 

2018

 

Pircs K*, Petri R*, Jakobsson J (2018) Crosstalk between MicroRNAs and Autophagy in Adult Neurogenesis: Implications for Neurodegenerative Disorders Brain Plasticity

doi: 10.3233/BPL-180066

 

2017

 

Drouin-Ouellet J*, Pircs K*, Barker AR, Jakobsson J, Parmar M (2017) Direct neuronal reprogramming for disease modeling studies using patient-derived neurons: What have we learned? Frontiers in Neuroscience

doi: 10.3389/fnins.2017.00530

Here we reviewed published literature on the work that has been undertaken using induced neurons (iNs) to model human brain disorders. As disease-modelling studies using direct neuronal reprogramming approach are becoming more widely adopted, we also define the criteria that are used to define the iNs, especially in relation to the extent to which they are mature adult neurons.

 

 

 

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